The International Complex Trait Consortium
A Genome Scan For Quantitative Trait Loci (QTLs)
Associated With
Aerobic Running Capacity In Rats
Lauren Gerard Koch, Justin A. Ways, George T. Cicila, Michael R. Garrett, Steven L. Britton
Functional
Genomics Laboratory, Medical College of Ohio, Toledo, OH 43614
ABSTRACT
The divide between health and disease is fundamentally defined within
variation for aerobic capacity. From a panel of inbred strains, we
identified Copenhagen (COP) and DA rats to be most divergent (145%) for
a test of aerobic treadmill running. An F2(COPxDA) intercross population
(n=224 males) was used to identify QTLs for aerobic running capacity.
Selective genotyping was done on F2(COPxDA) extremes (n=90) with 210
polymorphic microsatellite markers spaced 10-20cM apart. MapManagerQTX
detected QTL associations (LOD scores>1.85) on chromosomes 3, 7, 8, 16
and 20. Genotyping was completed on the entire F2(COPxDA) population
for these potential loci. Aerobic running capacity QTLs were
re-established for chromosomes 3 and 16. "Suggestive" linkage (LOD=2.2)
was observed near the p-terminus of Chr. 3 between D3Rat56 and D3Rat277.
The LOD plot on Chr. 16 revealed two peaks separated by more than 30
cM: D16Rat17 showed "significant" linkage (LOD=4.0), and D16Rat55 was
"suggestive" (LOD=2.9). All three QTLs showed a dominant mode of
inheritance where the presence of at least one DA-allele was associated
with greater distance run, compared to rats homozygous for the
COP-allele.
[Supported by NIH HL64270] Next Abstract: Genetic Models Of Aerobic Endurance Running Capacity In Rats